Headspace ethylene accumulation on Stizolobium hassjoo hairy root culture producing L-3,4-dihydroxyphenylalanine |
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Authors: | Lin-Shiang Sung Shih-Yow Huang |
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Institution: | (1) Department of Chemical Engineering, National Taiwan University, Taipei, 10617, Taiwan, Republic of China |
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Abstract: | The addition of 1-aminocyclopropane-1-carboxylic acid (ethylene precursor), or 2-chloroethylphosphonic acid (ethephon, an ethylene-releasing compound) decreased root dry weight and l-DOPA (l-3,4-dihydroxyphenylalanine) accumulation in hairy root cultures of Stizolobium hassjoo. The inhibition caused by ethephon-mediated ethylene release was alleviated by 0.5 mg CoCl2 l–1 as an inhibitor of ethylene biosynthesis. The action of ethylene was inhibited by 1.5 mg AgNO3 l–1. Ethylene thus lowers hairy root formation and l-DOPA production; CoCl2 decreases ethylene formation leading to a considerably improved root dry weight and l-DOPA production. |
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Keywords: | ethylene hairy root cultures l-DOPA Stizolobium hassjoo |
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