SARS冠状病毒M蛋白的生物信息学研究

针对GenBank上发布的来自不同国家地区的39条SARSCoV推测M蛋白,采用生物信息学软件分析其核酸和氨基酸序列,获得其分子生物学特征,确定突变位点,预测功能结构区、Motif及抗原决定簇,比较基因突变对这些功能结构的影响.结果表明:在39个病毒株M蛋白的666 bp中,共有18个病毒株在7个位点上发生了25次变异.在M蛋白序列上预测获得3个跨膜螺旋序列和一个可能的信号肽序列.氨基酸序列的变异主要发生在其跨膜和胞外区域,胞内区域相对较少.预测发现12个Motif和7个抗原决定簇.提示突变对M蛋白的结构功能区的影响不大,也未造成M蛋白的Motif的数量和构成发生改变.对抗原决定簇的影响也主要体现在序列成分构成的改变上,在设计疫苗时,应考虑由其导致的抗原特性改变.

Bioinformatic Analysis of Putative Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus

The objective of this paper was to find the biological characteristic of the SARS-CoV putative membrane protein and the variations among the sequence. The influence on the function and structure due to mutation were analyzed to provide theoretical basis for vaccine development. A series of bioinformatic software and tools were adopted to find and compare the quantity of variations among the sequences and predict the structural and functional sites, including motifs, signal peptides, low-complexity regions, transmembrane helix segments and antigenic determinants. The result showed that there are 25 variations occurred in 7 sites in 18 isolates. Three TMHs, one probable signal peptide sequence and totally 12 motifs as well as 7 antigenic determinants were predicted. M protein is a stable protein with weak hydrophilicity. The mutations occurred in the amino acid sequence make little influence on the structure and functional domain of the protein.;