Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate |
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Authors: | H. Zahradníčková P. Hartvich P. Šimek P. Hušek |
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Affiliation: | Biology Centre, Academy of Sciences of the Czech Republic, Laboratory of Analytical Biochemistry, Ceské Budejovice, Czech Republic. helenaz@bclab.eu |
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Abstract: | A novel sample preparation protocol for gas chromatographic (GC) analysis of amino acid enantiomers in peptides was developed. It comprises traditional acid hydrolysis, a novel treatment of the analytes with a fluoroalkyl chloroformate and GC/FID separation of enantiomers on a chiral capillary column. The major improvements consist in that the derivatization step proceeds in organic-aqueous media within seconds and the amino acid derivatives are volatile enough to suit the temperature range of the chiral Chirasil-Val capillary column. The approach was found beneficial for chiral analysis of pharmaceutically important Carbetocin peptide. |
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