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补料培养与溶氧控制联合应用对红豆杉细胞培养的影响
引用本文:罗杰,梅兴国.补料培养与溶氧控制联合应用对红豆杉细胞培养的影响[J].Acta Botanica Sinica,2002,44(11):1286-1290.
作者姓名:罗杰  梅兴国
作者单位:华中科技大学生命科学与技术学院 武汉430074 (罗杰),华中科技大学生命科学与技术学院 武汉430074(梅兴国)
基金项目:国家“九五”科技攻关重点项目 (96_c0 2_0 3_0 1),863计划项目 (10 2_12_0 6_0 1)~~
摘    要:为进一步提高红豆杉 (Taxuschinensis (Pilg.)Rehd .)细胞培养过程中紫杉醇的产量 ,采用细胞悬浮培养方法研究了补料培养与溶氧控制联合应用对紫杉醇产量的影响。 5L反应器中补料培养研究表明 ,培养过程中第 16天添加含 2 0g/L蔗糖的补料培养液有利于细胞的生长及紫杉醇的合成。 2 0L反应器中补料培养的研究结果表明 :2 0 %饱和度培养时紫杉醇含量最高 (0 .98mg/gDW) ,但 4 0 %~ 6 0 %溶氧饱和度能提高紫杉醇的产量。进一步研究表明 ,细胞在 6 0 %溶氧饱和度培养 2 0d后转入 2 0 %溶氧饱和度继续培养 12d ,能显著提高紫杉醇产量。补料培养与溶氧控制联合应用时 ,2 0L反应器中红豆杉细胞培养紫杉醇产量可达 18.7mg/L。

关 键 词:红豆杉  紫杉醇  补料培养  溶氧控制

Taxol Production in Suspension Cultures of Taxus chinensis as Affected by the Combination of Nutrient Feed with Dissolved Oxygen Control
Authors:LUO Jie  MEI Xing_Guo
Abstract:Influence of the combination of nutrient feed with dissolved oxygen control on taxol production in suspension cultures of Taxus chinensis (Pilg.) Rehd. was investigated in bioreactors. Addition of feeding medium with 20 g/L sucrose on day 16 enhanced both the biomass and taxol production in 5_L bioreactors. Further investigation of the fed_batch cultures in a 20_L bioreactor showed that cultivation under a low dissolved oxygen tension (DOT) of 20% for the entire culture resulted in the highest taxol content of 0.98 mg/g DW, while the taxol production was lower than that with 40% and 60% DOT. Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially under DOT of 60% for 20 d followed by changing the DOT to 20% for another 12 d. By combining the use of these two strategies, a maximum taxol content of 18.7 mg/L was obtained in a 20 L bioreactor.
Keywords:Taxus chinensis  taxol  nutrient feed  dissolved oxygen tension control
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