Substitution of inosine for yeastolate in the culture medium forDrosophila cells |
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Authors: | Tohru Marunouchi Tadashi Miyake |
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Institution: | (1) Laboratory of Cell Biology, Mitsubishi-Kasei Institute of Life Sciences, 11 Minamiooya, Machida, 194 Tokyo, Japan |
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Abstract: | Summary The nutritional requirement ofDrosophila cells (GM1 and GM2) was studied. TC Yeastolate contained in the medium forDrosophila cell culture was found to be replaceable with adenosine or inosine without appreciable changes in the generation time of
cells. The optimal concentration of either adenosine or inosine was 0.01 mM. Whereas adenosine manifested cell toxicity at
concentrations higher than 0.1 mM, in the case of inosine, such an inhibitory effect was not observed up to and at the concentration
of 1.0 mM. Further-more, the plating efficiency at cell densities as low as 2×103 cells per cm2 was raised from 0 to 10% by supplementing inosine (0.1 mM) for the TC Yeastolate. Therefore inosine is in practice more useful
than adenosine. Experiments using radioactive nucleosides suggested that both adenosine and inosine were exclusively incorporated
into RNA as adenosine-monophosphate. |
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Keywords: | Drosophila nutritional requirement inosine adenosine TC Yeastolate |
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