The evaluation of AKR leukemia virus purity: the requirement for both velocity and isopycnic centrifugation methods. |
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Authors: | C G Sciotto M Aikawa R R Kohn |
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Institution: | Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106 USA |
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Abstract: | The purity of AKR murine leukemia virus obtained by isopycnic centrifugation was compared with the purity obtained by combining velocity sedimentation and isopycnic centrifugation methods. Evaluation of AKR and Rauscher viral purity by electron microscopy and by analysis of 3H]uridine-labeled viral RNA demonstrated that the velocity centrifugation step is essential for the removal of contaminants banding in the viral density region (1.19 – 1.15 g/ml). For studies requiring relatively pure oncornavirus preparations, a combination of both velocity sedimentation and isopycnic centrifugation steps are suggested. Viral recovery of about 50% was obtained by the method described. |
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Keywords: | To whom correspondence should be addressed: Department of Pathology University of Colorado Medical Center Denver Colorado 80220 |
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