The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs |
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Authors: | Ian R McDonald JColin Murrell |
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Institution: | Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK |
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Abstract: | The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source. The resulting 525 bp PCR products were cloned and a representative number of clones were sequenced. Phylogenetic analysis of the derived amino acid sequences of the pmoA clones retrieved directly from environmental DNA samples revealed that they form a distinct cluster within representative PmoA sequences from type II methanotrophs and may originate from a novel group of acidophilic methanotrophs. The study also demonstrated the utility of the pmoA gene as a phylogenetic marker for identifying methanotroph-specific DNA sequences in the environment. |
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Keywords: | Methane monooxygenase gene Methanotroph Phylogenetic marker |
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