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Statistical analysis of kinetics,distribution and co-localisation of DNA repair foci in irradiated cells: Cell cycle effect and implications for prediction of radiosensitivity |
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| Authors: | Olga A Martin Alesia Ivashkevich Sharon Choo Lisa Woodbine Penny A Jeggo Roger F Martin Pavel Lobachevsky |
| Institution: | 1. Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia;2. Molecular Radiation Biology Laboratory, Research Division, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia;3. Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, VIC, Australia;4. Centre for Innate Immunity and Infectious Disease, Monash Institute for Medical Research, Monash University, Clayton, VIC, Australia1;5. Royal Children Hospital, Melbourne, VIC, Australia;6. Centre for Genome Damage and Stability, University of Sussex, Brighton BN1 9RQ, UK |
| Abstract: | Detection of γ-H2AX foci as a measure of DNA double strand break induction and repair provides the basis of a rapid approach to establish individual radiosensitivity. However, the assignment of criteria to define increased radiosensitivity is not straightforward. Experimental end points, analytical methods and proliferative status of the cells sampled for analysis are important. All these issues are addressed in the present study, which was prompted by a clinical request to assess the radiosensitivity status of an SCID paediatric patient being considered for bone marrow transplantation. |
| Keywords: | Gamma-H2AX foci Radiosensitivity Fibroblasts Cell cycle Repair kinetics Distribution 53bp1 Colocalisation SCID Artemis |
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