Matrix-induced fragmentation of P3'-N5' phosphoramidate-containing DNA: high-throughput MALDI-TOF analysis of genomic sequence polymorphisms |
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Authors: | Shchepinov M S Denissenko M F Smylie K J Wörl R J Leppin A L Cantor C R Rodi C P |
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Institution: | Mikhail S. Shchepinov, Mikhail
F. Denissenko, Kevin
J. Smylie, Ralf
J. Wörl, A. Lorieta Leppin, Charles
R. Cantor, and Charles
P. Rodi |
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Abstract: | Chemical and enzymatic approaches were used to produce polynucleotide fragments containing acid-labile internucleotide P3′-N5′ phosphoramidate bonds, either in a surface-bound form or in solution. The primer extension reaction utilizing 5′-amino-5′-deoxynucleoside 5′-triphosphates generates polynucleotides that can be fragmented into short, easy-to-analyze pieces simply by being premixed with the acidic matrices typically used for MALDI-TOF mass spectrometry of nucleic acids. This leads to detection procedures that are simple, robust and easy to automate. Utilizing this approach, a polymorphic site in the human ADRB3 gene was interrogated. Primer extensions with phosphoramidate analogs of dNTPs allowed for unambiguous discrimination of all possible genotypes. |
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