Origin of Embryo-Like Structures in Soybean Anther Culture Investigated Using SSR Marker |
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Authors: | Rodrigues Lia R Terra Tatiana de F Bered Fernanda Bodanese-Zanettini Maria Helena |
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Institution: | (1) Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Prédio 43323, CEP 91501-970 Porto Alegre-RS, Brasil |
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Abstract: | The Satt418 microsatellite locus was examined in order to investigate the origin of embryo-like structures (ELS) obtained from soybean anther culture. Four heterozygous plants were used as anther donors. A total of 7000 anthers were placed on the induction medium under culture conditions known to trigger androgenic response. After 60 days of culture, upper portion of 216 ELS were carefully removed and transferred to a proliferation medium, in order to obtain sufficient tissue for DNA extraction. Callogenic masses originated from 114 ELS were screened for the Satt418 microsatellite locus. Heterozygous and homozygous ELS were identified, suggesting occurrence of somatic embryogenesis and androgenesis in the same system. This unexpected morphogenic response seems to be genotype-dependent. |
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Keywords: | androgenesis anther culture Glycine max microsatellite somatic embryogenesis |
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