Immunohistochemical localisation of TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney |
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Authors: | Lina Gubhaju Andrew Laslett John F Bertram Anthony Zulli M Jane Black |
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Institution: | (1) Department of Anatomy and Developmental Biology, Monash University, P. O. Box 13C, Clayton, VIC, 3800, Australia;(2) Australian Stem Cell Centre, Monash University, Clayton, VIC, 3800, Australia;(3) Department of Cardiology, University of Melbourne, Austin Health and Northern Health, Heidelberg, VIC, 3084, Australia |
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Abstract: | The baboon is an ideal animal model to study human kidney development. The aim of the current study was to use immunohistochemistry
to localise the antigens TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney where nephrogenesis was
still on-going and in kidneys where nephrogenesis was complete. Fixed kidney sections from baboons delivered at 125, 140,
175 and 185 days gestation (term = 185 days) were immuno-labelled with antibodies directed against TRA-1-60, TRA-1-81, GCTM-2
and podocalyxin. In kidneys with on-going nephrogenesis (125 and 140 days gestation), TRA-1-60, TRA-1-81 and GCTM-2 were specifically
localised to the apical plasma membrane of the epithelium of the ureteric ampullae and the collecting ducts, while podocalyxin
immunostaining was not detected. In kidneys where nephrogenesis was complete (175 and 185 days gestation) localisation of
these markers was again very specifically localised to the collecting ducts. In conclusion, although further experimentation
is required to confirm the identity of the specific cell types marked by these antibodies, this study provides new insight
into the distribution of commonly utilised stem cell antibodies in the developing baboon kidney. |
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Keywords: | Kidney Nephrogenesis Baboon Stem cells |
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