Disruption of sucA, which encodes the E1 subunit of alpha-ketoglutarate dehydrogenase, affects the survival of Nitrosomonas europaea in stationary phase |
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Authors: | Hommes Norman G Kurth Elizabeth G Sayavedra-Soto Luis A Arp Daniel J |
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Affiliation: | Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley, Corvallis, OR 97331-2902, USA. |
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Abstract: | Although Nitrosomonas europaea lacks measurable alpha-ketoglutarate dehydrogenase activity, the recent completion of the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the E1 subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase. |
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