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Identification of Microorganisms by Liquid Chromatography-Mass Spectrometry (LC-MS1) and in Silico Peptide Mass Libraries
Authors:Peter Lasch  Andy Schneider  Christian Blumenscheit  Joerg Doellinger
Affiliation:Robert Koch-Institute, ZBS6, Proteomics and Spectroscopy, Berlin, Germany
Abstract:
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  • Highlights
    • •Rapid identification of bacteria by LC-MS1 and in silico databases.
    • •Pattern analysis-based strategy for identifying bacteria by experimental LC-MS1 data.
    • •Compilation of an in silico database of taxon-specific tryptic peptide mass profiles.
    • •Tests of the identification pipeline with LC-MS1 data from bacteria.
    Keywords:Bacteria  microbiology  bioinformatics software  diagnostics  mass spectrometry  identification of microorganisms  LC-MS1  diagnostic
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