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High-dimensional Cytometry (ExCYT) and Mass Spectrometry of Myeloid Infiltrate in Clinically Localized Clear Cell Renal Cell Carcinoma Identifies Novel Potential Myeloid Targets for Immunotherapy
Affiliation:1. Medical Scientist Training Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;2. Graduate Program in Immunology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;3. Bloomberg-Kimmel Institute for Immunotherapy, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;4. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;5. Pathobiology Graduate Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;6. Department of Oncology, Johns Hopkins School of Medicine and The Sidney Kimmel Comprehensive Cancer Center, Baltimore, Maryland, USA;7. Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;8. The James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Abstract:
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  • Highlights
    • •Using ExCYT, genomics, and Mass Spectrometry, we were able to uncover immune cell marker alterations that provide new insight into the biology of early stage ccRCC.
    • •Among the CD45+ population, we observed a high level of myeloid cell infiltration in treatment-naïve ccRCC tissues.
    Keywords:immunotherapy  monocytes  macrophages  peripheral blood mononuclear cells  tumor microenvironment  ExCYT  kidney cancer  immunology  clinical proteomics  mass spectrometry  clear cell renal cell carcinoma
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