Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search

Institution:	3. Laboratory of Protein and Peptide Pharmaceuticals & Proteomics Laboratory, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;4. National Center for Mathematics and Interdisciplinary Sciences, Key Laboratory of Random Complex Structures and Data Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100190, China;5. Center for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, Louisiana 70112;6. Computer Network Information Center, Chinese Academy of Sciences, Beijing 100101, China;12. Research Center for Basic Sciences of Medicine, Basic Medical College, Guizhou Medical University, Guiyang 550025, China

Abstract:	Download : Download high-res image (200KB) Download : Download full-size image Highlights •This study proposed a spectral library search method to accurately identify N-linked glycopeptides in human serum through LC-MS/MS with pMatchGlyco software. •The identification depth of serum N-linked intact glycopeptides and glycoproteins was increased by combination of acetonitrile precipitation, HILIC enrichment and high-pH RPLC fractionation. •22,677 unique serum N-linked intact glycopeptides corresponding to 526 N-linked glycoproteins were identified with N-glycosylation motif-specific FDR control. •This study revealed the great microheterogeneity of N-linked glycoproteins in serum.

Keywords:	Glycomics glycoproteomics bioinformatics software mass spectrometry glycoprotein pathways glycoproteins co-elution human serum isotopic distribution N-linked intact glycopeptide spectral library search
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