Accurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Accurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease

Authors:	zge Karayel Francesca Tonelli Sebastian Virreira Winter Phillip E Geyer Ying Fan Esther M Sammler Dario R Alessi Martin Steger Matthias Mann

Institution:	1. Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany;2. Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee, United Kingdom;3. Department of Neurology, School of Medicine, Ninewells Hospital, Ninewells Drive, Dundee, United Kingdom

Abstract:	Download : Download high-res image (63KB) Download : Download full-size image Highlights ?MS-based clinical assay that accurately determines phospho Rab10 occupancy. ?Stable isotope labeled phosphopeptide injected as a standard with endogenous tryptic phospho Rab peptide for accurate ratio determination. ?Determination of pRab levels in neutrophils of Parkinson disease patients. ?Relevance of pRab levels as marker of PD.

Keywords:	Biomarker: diagnostic clinical proteomics neurodegenerative diseases parallel reaction monitoring Parkinson disease phosphorylation protein kinases targeted mass spectrometry targeted therapies selected ion monitoring
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