Improving Identification of In-organello Protein-Protein Interactions Using an Affinity-enrichable,Isotopically Coded,and Mass Spectrometry-cleavable Chemical Crosslinker
Institution:
3. Department of Biochemistry and Microbiology, University of Victoria, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada;4. University of Victoria - Genome British Columbia Proteomics Centre, #3101-4464 Markham Street, Vancouver Island Technology Park, Victoria, BC V8Z7X8, Canada;5. Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands;6. Leibniz Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany;12. Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Freiburg, Germany;8. Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Germany;9. Gerald Bronfman Department of Oncology, Jewish General Hospital, Montreal, Quebec, H3T 1E2, Canada;10. Department of Data Intensive Science and Engineering, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Nobel St., Moscow 143026, Russia
Abstract:
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Highlights
•Used affinity-enrichable, isotopically coded, and MS-cleavable crosslinker.
•Targeted acquisition strategy based on isotopic-coding described and evaluated.
•Novel data analysis pipeline developed provides improved crosslink identification.
•Large dataset reveals hundreds of mitochondrial protein-protein interactions.
