Acquiring and Analyzing Data Independent Acquisition Proteomics Experiments without Spectrum Libraries期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

Acquiring and Analyzing Data Independent Acquisition Proteomics Experiments without Spectrum Libraries

Institution:	1. Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, USA;2. Proteome Software, Inc. Portland, Oregon, USA;3. Department of Genome Sciences, University of Washington, Seattle, Washington, USA;4. Institute for Systems Biology, Seattle, Washington, USA

Abstract:	Download : Download high-res image (144KB) Download : Download full-size image Highlights •Rapid DIA-only library building with gas-phase fractionation. •Recommended DIA acquisition strategies with staggered windows and forbidden zones. •Optimized DIA instrument settings for several Thermo Orbitrap instruments. •Data analysis tutorial using open source DIA software.

Keywords:	Data evaluation label-free quantification mass spectrometry protein identification quantification data independent acquisition DIA
本文献已被 ScienceDirect 等数据库收录！