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1,3-二氯苯对芦苇湿地土壤生物活性的影响
引用本文:梁慧星,陈爱辉,丁成,李朝霞,吴小芹. 1,3-二氯苯对芦苇湿地土壤生物活性的影响[J]. 微生物学通报, 2011, 38(4): 569-574
作者姓名:梁慧星  陈爱辉  丁成  李朝霞  吴小芹
作者单位:1. 南京林业大学森林资源与环境学院,江苏,南京,210000;盐城工学院环境科学与工程学院,江苏,盐城,224051
2. 盐城工学院环境科学与工程学院,江苏,盐城,224051
3. 南京林业大学森林资源与环境学院,江苏,南京,210000
基金项目:国家自然科学基金资助项目(No. 20607018); 江苏省自然科学基金项目(No. BK2009171); 盐城市农业科技发展项目(No. YK2009066); 盐城工学院应用化学重点学科基金项目(No. XKY2010002)
摘    要:采用传统微生物培养及酶学方法,研究了1,3-二氯苯(1,3-DCB)对芦苇湿地土壤微生物及酶活的影响。结果表明,1,3-DCB处理芦苇湿地土壤对微生物不同类群影响不同,其中对细菌和放线菌生长具有抑制作用,各浓度1,3-DCB处理对放线菌的抑制较为显著,浓度高于120μg/g显著抑制对细菌的生长。1,3-DCB对真菌表现为先刺激后抑制。在实验过程中,1,3-DCB可显著抑制湿地土壤过氧化氢酶和多酚氧化酶的活性,表明这2种酶是1,3-DCB污染芦苇湿地土壤的敏感指标。

关 键 词:1  3-二氯苯  微生物种群  过氧化氢酶  多酚氧化酶

Influences of 1,3-dichlorobenzene on biological activity in reed wetland soil
LIANG Hui-Xing,CHEN Ai-Hui,DING Cheng,LI Zhao-Xia and WU Xiao-Qin. Influences of 1,3-dichlorobenzene on biological activity in reed wetland soil[J]. Microbiology China, 2011, 38(4): 569-574
Authors:LIANG Hui-Xing  CHEN Ai-Hui  DING Cheng  LI Zhao-Xia  WU Xiao-Qin
Affiliation:1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, Jiangsu 210000, China; 2. School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China;2. School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China;2. School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China;2. School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China;1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, Jiangsu 210000, China
Abstract:With traditional microbiological culture and enzyme method,the effect of 1,3-dichlorobenzene(1,3-DCB) on reed wetland soil microbial and enzyme activities was studied.Results demonstrated that different groups of oil microorganisms have different responses to 1,3-DCB,in which the actinomycetes was significantly inhibited.When the 1,3-DCB concentration was higher than 120 μg/g,the bacterial amounts were significantly inhibited.The soil fungi amounts were initially increased at the beginning of the experiment but inhibited with time.During the experiment,1,3-DCB treatment could significantly inhibit the soil catalase and polyphenol oxidase activity.Results indicated that these two enzymes are sensitive to 1,3-DCB contamination.
Keywords:1  3-dichlorobenzene   Microbial community   Catalase   Polyphenol oxidase
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