Isolation and characterization of an ipt gene from the Ti plasmid Bo542 |
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| Authors: | Timothy J Strabala Sebastian Y Bednarek Gregory Bertoni and Richard M Amasino |
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| Institution: | (1) Department of Biochemistry, University of Wisconsin-Madison, 420 Henry Mall, 53706 Madison, WI, USA;(2) Present address: MSU-DOE Plant Research Laboratory, Michigan State University, 48824 East Lansing, MI, USA |
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| Abstract: | Summary A 1.9 kb clone of the T-DNA region of the Agrobacterium tumefaciens Ti plasmid Bo542 which exhibited homology to the isopentenyl transferase (ipt) locus of pTiA6 was identified by low stringency DNA hybridization. Introduction of this segment of pTiBo542 DNA into cells of Nicotiana tabacum or N. glauca caused tumor formation in vivo, and allowed hormone independent growth in vitro. Furthermore, this DNA segment complemented ipt mutant strains of A. tumefaciens, restoring their ability to cause tumors on Kalanchöe leaves and tomato stems. The complete DNA sequence of this segment has been determined, revealing an open reading frame homologous to other known Agrobacterium ipt genes. |
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| Keywords: | Agrobacterium tumefaciens Ti plasmid T-DNA Isopentenyl transferase Cytokinin |
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