Effect of sorbitol concentration on regeneration of embryogenic calli in upland rice varieties (Oryza sativa L.) |
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Authors: | Pengpeng Geng Honggui La Huaqi Wang Elliot J C Stevens |
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Institution: | (1) Upland Rice Research Center, College of Agronomy and Biotechnology, China Agricultural University, YuanMingYuan West Road 2, Beijing, 100094, P.R. China;(2) Key Laboratory of Crops Genomic & Genetic Improvement, Ministry of Agriculture, the People’s Republic of China, Beijing, 100094, P.R. China;(3) Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, USA;(4) The University of Newcastle, NSW, 2260, Australia |
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Abstract: | This study describes the impact of sorbitol on plantlets regeneration frequency (PRF) of four rice cultivars (japonica, Oryza sativa L.) both of which mature and immature embryo-derived calli were investigated. The variance analysis results showed that PRF
of the three elite upland rice cultivars, Handao297 (HD297), Handao502 (HD502), Handao65 (HD65) and one lowland rice cultivar
Zhongzuo93 (ZZ93) were significantly increased with addition of appropriate amount of sorbitol in culture media. Supplementing
appropriate sorbitol in the media of a continous culture from induction and maintenance to regeneration for mature embryo-derived
calli could improve PRF dramatically, originally from 27.6% up to a maximum of 71.8%. Especially to low regenerative capacity
(LRC) cultivar HD65, the PRF was increased over 7-fold (from 9.7% to 74.0%). The optimum concentrations of sorbitol for calli
induction, subculture and differentiation were 5, 20 and 40 g/l, respectively. Adding sorbitol, only in maintenance media
at concentration of 20 g/l, also enhanced the PRF greatly in all the cultivars from 27.6% to 43.3%. Similar results were observed
when incorporating with maltose in regenerating media both in immature and mature embryo-derived calli. The optimal concentration
was 25 g/l sorbitol + 20 g/l maltose and 20 g/l sorbitol + 25 g/l maltose, respectively. HD297 appeared to be the most responsive
genotype compared to other cultivars in PRF, 99.2% in immature embryo-derived calli and 76.8% in mature embryo-derived calli.
The results and relevant conclusions might be valuable to establish an efficient plant regeneration system from somatic embryogenesis
culture in upland rice. |
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Keywords: | Sorbitol Plantlet regeneration frequency (PRF) Embryogenic callus Upland rice cultivar |
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