Mineralization of 4-sulfophthalate by aPseudomonas strain isolated from the River Elbe |
| |
Authors: | Monika Richter Rolf-Michael Wittich |
| |
Institution: | (1) Abteilung für Mikrobiologie, Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-2000 Hamburg 52, F.R. Germany;(2) Present address: Bereich Mikrobiologie, Gesellschaft für biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany |
| |
Abstract: | The bacteriumPseudomonas sp. strain RW31 isolated from the river Elbe utilized the ammonium salt of 4-sulfophthalate (4SPA) as sole source of carbon, sulfur, nitrogen, and energy and grew also with phthalate (PA) and several other aromatic compounds as sole carbon and energy source. The xenobiotic sulfo group of 4SPA was eliminated as sulfite, which transiently accumulated in the culture supernatant up to about 10 µM and was slowly oxidized to the stoichiometrical amount of sulfate. Biodegradation routes of 4SPA as well as of PA converged into the protocatechuate pathway and from found activities for the decarboxylation of 4,5-dihydroxyphthalate we deduce this compound the first rearomaticized intermediate after initial dioxygenation. Protocatechuate then underwentmeta-cleavage mediated by a protocatechuate 4,5-dioxygenase activity which was competitively inhibited by the structurally related compound 3,4,5-trihydroxybenzoate; protocatechuate accumulated in the medium up to an about 2 mM concentration. Indications for the presence of selective transport systems are presented. |
| |
Keywords: | decarboxylation desulfonation 4 5-dihydroxyphthalate meta-cleavage protocatechuate pathway Pseudomonas sp RW31 4-sulfophthalate mineralization |
本文献已被 SpringerLink 等数据库收录! |
|