Use of bitter gourd (Momordica charantia) peroxidase together with redox mediators to decolorize disperse dyes |
| |
Authors: | Rukhsana Satar Qayyum Husain |
| |
Institution: | (1) Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India |
| |
Abstract: | In this study, salt fractionated bitter gourd (Momordica charantia) peroxidase was used for the decolorization of water-insoluble disperse dyes; Disperse Red 17 and Disperse Brown 1. Effect
of nine different redox mediators; bromophenol, 2,4-dichlorophenol, guaiacol, 1-hydroxybenzotriazole, m-cresol, quinol, syringaldehyde,
violuric acid, and vanillin on decolorization of disperse dyes by bitter gourd peroxidase has been investigated. Among these
redox mediators, 1-hydroxybenzotriazole was the most effective mediator for decolorization of both the dyes by peroxidase.
Bitter gourd peroxidase (0.36 U/mL) could decolorize Disperse Red 17 maximally 90% in the presence of 0.1 mM 1-hydroxybenzotriazole
while Disperse Brown 1 was decolorized 65% in the presence of 0.2 mM 1-hydroxybenzotriazole. Maximum decolorization of these
dyes was obtained within 1 h of incubation at pH 3.0 and temperature 40°C. The application of such enzyme plus redox mediator
systems may be extendable to other recalcitrant and water insoluble synthetic dyes using novel redox mediators and peroxidases
from other new and cheaper sources. |
| |
Keywords: | decolorization disperse dyes 1-hydroxybenzotriazole Momordica charantia peroxidise redox mediators |
本文献已被 SpringerLink 等数据库收录! |
|