Short communication: Production of l-glutamate oxidase by Streptomyces sp. N1 in submerged fermentation |
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| Authors: | Q-S Li J-J Zhong |
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| Institution: | (1) Research Institute of Biochemical Engineering, 130 Meilong Road, 2000237 Shanghai, China;(2) State Key Laboratory of Bioreactor Engineering at the East China University of Science and Technology, 130 Meilong Road, 2000237 Shanghai, China |
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| Abstract: | In submerged fermentation of Streptomyces sp. N1 in a shake flask, glucose (3% w/v) and (NH4)2SO4 (0.6% w/v) were found to be suitable for extracellular l-glutamate oxidase (GluOx) (EC.1.4.3.11) production. GluOx production was higher with the addition of further KCl or MgCl2 to the medium within the range of 0 to 0.12% (w/v). The effect of inoculum type, that is, spore inoculation or mycelium inoculation on GluOx biosynthesis was also investigated, and the maximum GluOx production obtained was 2.7 U/ml after 33h fermentation with mycelium inoculation. The results demonstrated a much higher GluOx production and productivity compared with those reported previously. |
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| Keywords: | l-glutamate oxidase" target="_blank">Extracellular l-glutamate oxidase inoculum type medium components metabolism regulation Streptomyces sp submerged fermentation |
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