Amino-terminal fragment of urokinase-type plasminogen activator inhibits HIV-1 replication |
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Authors: | Wada M Wada N A Shirono H Taniguchi K Tsuchie H Koga J |
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Institution: | Laboratories for Bioengineering and Research, JCR Pharmaceuticals Company, Ltd., 2-2-10 Murotani, Nishi-ku, Kobe, 651-2241, Japan. wada-m@jcrpharm.co.jp |
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Abstract: | CD8+ T lymphocytes have been shown to produce unidentified soluble factors active in suppressing HIV-1 replication. In this study, we purified an HIV-1 suppressing activity from the culture supernatant of an immortalized CD8+ T cell clone, derived from an HIV-1 infected long-term nonprogressor, and identified this activity as the amino-terminal fragment (ATF) of urokinase-type plasminogen activator (uPA). ATF is catalytically inactive, but suppresses the release of viral particles from the HIV-1 infected cell lines via binding to its receptor CD87. In contrast, cell proliferation and the secretion of an HIV-1 LTR driven reporter gene product were not affected by ATF. These findings suggest that ATF may inhibit the assembly and budding of HIV-1, which provides a novel therapeutic strategy for AIDS. |
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