Establishment and in vitro regeneration studies of the potential oil crop species Camelina sativa |
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Authors: | Tattersall Alex Millam Steve |
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Institution: | (1) Crop Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom |
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Abstract: | Camelina sativa was successfully established in vitro and systems for the regeneration of shoots from leaf explants developed.
Methods for the surface-sterilisation of seeds were used which gave 95% germination, though the in vitro grown seedlings failed
to develop beyond 28 days culture. In a micropropagation system, the rooting response of nodal explants was increased from
a control level of 26.4% to 46.7% by the addition of 5.4 μM NAA. Leaf explants were more efficient for the regeneration of
root and shoots than hypocotyls. For regeneration from leaf tissue the use of auxin (NAA) alone in the medium above a level
of 0.54 μM resulted in root or callus growth. Cytokinin, in the form of BA alone failed to induce regeneration, but a combination
of 4.44 μM BA and 0.54 μM NAA induced shoot regeneration at rates over 10.0 shoots per explant. Regenerated shoots were successfully
transplanted to soil and flowered and set seed normally.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Camelina sativa tissue regeneration eicosenoic acid |
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