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Improved alpha-glycerophosphate dehydrogenase assay system suitable for continuous recording |
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| Authors: | B C Hummel P G Walfish |
| Affiliation: | 1. Department of Biochemistry, Baylor College of Medicine, Houston, Texas, 77030 USA;2. Laboratory of Parasitology, University of Leiden, Leiden, The Netherlands;3. Laboratory of Histochemistry and Cytochemistry, University of Leiden, Leiden, The Netherlands |
| Abstract: | An automated method for measurement of proteinase activities using fluorogenic substrates is described. Enzyme assays were performed in polystyrene microtitration trays as normally used for the enzyme-linked immunosorbent assay technique. The reaction products were measured using an inverted fluorescence microscope equipped with a photometer. Data acquisition and processing were via a microcomputer. An acidic thiol-dependent proteinase was used to illustrate the utility of this technique. |
| Keywords: | To whom correspondence should be addressed. |
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