Use of a colorimetric system to detect enzymes expressed by germinating conidia of entomopathogenic fungi |
| |
Authors: | G. N. El-Sayed C. M. Ignoffo T. D. Leathers S. C. Gupta |
| |
Affiliation: | (1) Biological Control of Insects Research Laboratory, USD A/ARS, P.O. Box 7629, 65205 Columbia, MO, USA;(2) Microbial Properties Research Center, USDA/ARS, 1815 N. University Street, 61604 Peoria, IL, USA |
| |
Abstract: | An apiZYM system, with 19 substrates, was used to detect enzymes expressed by germinating conidia of Nomuraea rileyi (5 isolates), Nomuraea atypicola, Nomuraea anemonoides, Beauveria bassiana and Metarhizium anisopliae. Similar enzyme profiles were obtained for two of the N. rileyi isolates (Mississippi, Ecuador) regardless of whether culture medium (Sabouraud-maltose-yeast) or cuticle (from larvae of Trichoplusia ni, Heliothis zea or Heliothis virescens) were used as substrates. Centroid-clustering analysis revealed three distinct enzyme profiles.Mention of a proprietary product in this paper does not consitute a recommendation for use by USDA. |
| |
Keywords: | Beauveria biochemistry enzyme profiles entomopathogenic fungi insects Metarhizium Nomuraea apiZYM system |
本文献已被 SpringerLink 等数据库收录! |
|