Production of ethanol in repeated-batch fermentation with membrane-type bioreactor |
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Institution: | 1. Univ. Bordeaux, LaBRI, UMR 5800, PICTURA, Talence F-33400, France;2. CNRS, LaBRI, UMR 5800, PICTURA, Talence F-33400, France;3. Univ. Bordeaux, IMB, UMR 5251, Talence F-33400, France;4. CNRS, IMB, UMR 5251, Talence F-33400, France;5. Bordeaux INP, LaBRI, UMR 5800, PICTURA, Talence F-33400, France |
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Abstract: | The average ethanol content in sake is 14 wt%; continuous production of such a high ethanol content was found not to be stably maintained in a packed-bed bioreactor with immobilized yeast cells, used normally for production of an ethanol content of up to 10 wt%. However, use of repeated-batch ethanol fermentation incorporating a membrane filter for product separation enabled a high ethanol content and improved productivity to be achieved. In this bioreactor, the yeast cells were retained within the bioreactor and a high yeast concentration was possible. A filtrate containing 14 wt% ethanol was obtained steadily after each batchwise operation. At a yeast concentration of 110 g/l, an ethanol productivity of 3.5 g/l/h was attained, which is 9 times higher than that in conventional batch fermentation. A mathematical model is proposed for assessment of the repeated-batch fermentation process. The estimated results agreed well with the observed ones. With a view to the application of this system to sake production, the aroma components of the filtrate were assayed and compared with those of a commercial-grade sake. |
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