Tachykinin immunoreactivity in the european common frog,Rana temporaria: Localization,quantification and chromatographic characterization |
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| Institution: | 1. Division of Cell and Experimental Biology, School of Biology and Biochemistry The Queen''s University of Belfast, Belfast BT7 1NN, U.K.;2. Department of Medicine, The Queen''s University of Belfast, Belfast BT7 1NN, U.K.;1. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;2. Key Laboratory for Tropical Marine Fishery Resource Protection and Utilization of Hainan Province, Hainan Tropical Ocean University, Sanya 570228, China;1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;2. Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;1. College of Fisheries, Huazhong Agriculture University, Wuhan, Hubei, 430070, China;2. Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei 443100, China;3. Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei 443100, China |
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| Abstract: | 1. Tachykinin immunoreactivity has been localized, quantified and chromatographicallycharacterized in the brain, stomach, intestine and skin of Rana temporaria.2. Antisera to mammalian substance P (SP) and neurokinin A (NKA) immunostained nerve fibres in all tissues except skin, and a population of mucosal endocrine cells in the intestinal epithelium.3. Radioimmunoassay of tissue extracts identified SP immunoreactivity in all tissues but NKA immunoreactivity was restricted to the brain.4. Chromatographie analysis of both frog tachykinins revealed that they possessed different physicochemical properties than their mammalian counterparts. |
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