The enzymatic acyl exchange of phospholipids with lipases |
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| Institution: | 2. University College, Cork, Ireland;1. Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt;2. Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt;3. Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt;1. Department of Biochemistry & Molecular Biology, Indiana University School of Medicine-South Bend, South Bend, IN, USA;2. Department of Chemistry & Biochemistry, University of Notre Dame, Notre Dame, IN, USA |
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| Abstract: | The acyl exchange of phospholipids with lipases was investigated. The lipase from Rhizopus delemar catalyzed the acyl exchange reaction between various phospholipids and fatty acids. When we incubated 1,2-dipalmitoyl-sn-glycero-3-phosphatidyl choline (DPPC) and oleic acid with lipase from R. delemar, the yield of diacyl phosphatidyl choline (PC) was 25% and the fatty acid composition of the converted PC was an oleic acid content of 25% and a palmitic acid content of 75%. This reaction exhibited 1-positional specificity. Three industrial lipases from Rhizopus sp., Mucor javanicus, and Candida cylindracea had the activity of the acyl exchange of phosphatidyl choline. The lipase from R. sp. gave the best result. |
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