Continuous production of 4-ethylguaiacol by immobilized cells of salt-tolerant Candida versatilis in an airlift reactor |
| |
| Affiliation: | 1. Research Division, Kikkoman Corporation, Noda-shi, Chiba 278, Japan;1. Division of Advanced Materials Engineering, Kongju National University, 1223–24, Cheonan-daero, Cheonan, Chungnam, 31080, South Korea;2. Department of Chemical & Biological Engineering, Hanbat National University, 125, Dongseodaero, Yuseong-gu, Daejeon, 34158, South Korea;3. Department of Materials Science and Chemical Engineering, Hanyang University, 55, Hanyangdaehak-ro, Sangrok-gu, Ansan, Gyeonggi-do, 15588, South Korea;1. School of food Science and Engineering, South China University of Technology, Guangzhou 510640, China;2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 10090, China;1. Procter & Gamble Service GmbH, 65824 Schwalbach am Taunus, Germany;2. Compusense Inc., Guelph, Ontario, Canada;3. Qi Statistics Ltd, Reading, UK;1. University of Novi Sad, Institute of Food Technology, Bul. cara Lazara 1, 21000, Novi Sad, Serbia;2. University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11120, Belgrade, Serbia;3. University of Belgrade, Faculty of Agriculture, Department for Agrobotany, Nemanjina 6, 11080, Belgrade, Serbia |
| |
| Abstract: | The effects of pH, temperature, aeration, and residence time on the continuous production of 4-ethyl-guaiacol (4-EG), which is one of the characteristic aroma components in soy sauce, by immobilized cells of the salt-tolerant yeast Candida versatilis were investigated using an airlift reactor. The optimum pH and temperature were about 4.0 and 30–33°C, respectively. The amount of 4-EG in the liquid was constant even during alterations of nitrogen/air ratio in the supplied gas. A large amount of 4-EG (over 20 ppm) was produced at a residence time from 5 to 28 h and 1–3 ppm of 4-EG, which was the optimum concentration in conventional soy souce, was produced at a shorter residence time of 0.5 h. The 4-EG production by immobilized C. versatilis cells using the airlift reactor was stable for 40 d. It was found that the immobilized cell method was effective for the production of 4-EG by C. versatilis cells. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
