An optimized antibody-chelator conjugate for imaging of carcinoembryonic antigen with indium-111 |
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| Institution: | 1. Abbott Laboratories, Abbott Park, IL 60064 U.S.A.;2. Condell Memorial Hospital, Libertyville, IL 60048, U.S.A.;1. Spin-Optoelectronics and Ferro-Thermoelectric (SOFT) Materials and Devices Laboratory, Department of Physics, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan;2. Department of Physics, College of Sciences, Princess Nourah bint Abdulrahman University, P. O. Box 84428, Riyadh, 11671, Saudi Arabia;3. Department of Chemistry, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan;4. Department of Physics, Faculty of Science, King Khalid University, P. O. Box 9004, Abha, Saudi Arabia;5. Laboratoire de Physique de la Matière Condensée, Département de Physique, Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, 1060, Tunis, Tunisia;1. Mechanical and Materials Engineering Department, Worcester Polytechnic Institute, 100 Institute Rd, Worcester, MA, 01609, USA;2. Department of Materials Science and Engineering, Pennsylvania State University, University Park, PA, 16802, USA;1. Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran;2. Textile Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran;1. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China;2. Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Department of Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China;3. Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310058, China;1. Department of Industrial and Materials Science, Chalmers University of Technology, Gothenburg, Sweden;2. Safran Aircraft Engines, Vernon, France |
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| Abstract: | A monoclonal antibody to carcinoembryonic antigen showing minimal cross-reactivity with blood cells and normal tissues was derivatized with benzylisothiocyanate derivatives of EDTA and DTPA. Seven chelators per immunoglobulin could be incorporated without loss of immunoreactivity. The resulting conjugates, labeled with indium-111, showed low liver uptake in animals. A cold kit, comprising the DTPA conjugate at a molarity of antibody bound chelator exceeding 1 x 10−4M, gave radiochemical yields of indium labeled antibody of ⩾95% and was stable for 1 yr. |
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