Purification of staphylococcal Protein A by affinity precipitation: dissociation of Protein A from the adsorbent with chemical reagents |
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| Affiliation: | 1. Howard P. Isermann Department of Chemical & Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA;2. Center for Biotechnology & Interdisciplinary Studies (CBIS), Rensselaer Polytechnic Institute, Troy, NY, USA |
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| Abstract: | Some other ways to elute Protein A adsorbed on an adsorbent for the affinity precipitation process than desorption with a glycine-HCl buffer (pH 2.5) are examined. Protein A was recovered at a satisfactory yield by dissociation with a 3.5 M KSCN solution without exposure to extreme low pH. |
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