Enzymatic production of tryptophan using a lipoic acid and thiamine double auxotroph of Enterobacter aerogenes having both pyruvic acid productivity and high tryptophanase activity |
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| Affiliation: | 1. American Hip Institute Research Foundation, Chicago, Illinois, U.S.A.;2. American Hip Institute, Chicago, Illinois, U.S.A.;1. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China;2. Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China;1. Soil Science Department, Faculty of Agriculture, Zagazig University, 44511 Zagazig, Egypt;2. Agricultural Botany Department, Faculty of Agriculture, Zagazig University, 44511 Zagazig, Egypt;3. Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt;4. Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi Arabia;5. Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;6. Plant Production Department, Arid Lands Cultivation Research Institute, the City of Scientific Research and Technological Applications, SRTA-City, Borg El Arab, Alexandria, Egypt;7. Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, 44511 Zagazig, Egypt;1. École Supérieure en Sciences Biologiques d''Oran (ESSBO), BP 1042, SAIM MOHAMED 31003 Oran, Algeria;2. Laboratoire d’Aquaculture et de Bioremédiation (AQUABIOR), Université d''Oran 1 Ahmed BENBELLA, B.P 1524, El M''Naouer - 31000 Oran, Algérie |
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| Abstract: | Washed cells of a newly-developed double auxotroph for lipoic acid and thiamine, Enterobacter aerogenes LT-94, produced about 30 g/l of pyruvic acid from 5% glucose, and subsequently converted the acid to tryptophan in a yield of 16.7 g/l by the reverse reaction of tryptophanase, which had been induced under derepressed conditions. These yields were higher than those of the parent strain, L-12, showing only lipoic acid auxotrophy. |
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