Cutibacterium avidum is phylogenetically diverse with a subpopulation being adapted to the infant gut |
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| Institution: | 1. Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH-Zurich, 8092 Zurich, Switzerland;2. Division of Gastroenterology and Nutrition, University Children’s Hospital Zurich, 8032 Zurich, Switzerland;3. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Czech Republic;4. Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcká 129, Prague 6, Suchdol 165 00, Czech Republic;2. Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul 151-742, Republic of Korea;4. Department of Beauty Care, College of Medical Science, Deagu Haany University, Gyeongsan 38610, Republic of Korea;1. Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23507, USA;2. Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA;1. Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614 Poznan, Poland;2. Poznan Science and Technology Park, Adam Mickiewicz University Foundation, Rubiez 46, 61-612 Poznan, Poland;3. West Pomeranian University of Technology in Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Microbiology and Biotechnology, Piastów Avenue 45, 70-311 Szczecin, Poland;1. Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal;2. Maurice Müller Laboratories (DBMR), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, 3008 Bern, Switzerland;3. Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital Friedrich-Schiller University, 07747 Jena, Germany;4. Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisbon, Portugal;5. Departamento de Biologia Animal, Centro de Ecologia, Evolução e Alterações Ambientais, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisbon, Portugal;6. Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA |
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| Abstract: | The infant gut harbors a diverse microbial community consisting of several taxa whose persistence depends on adaptation to the ecosystem. In healthy breast-fed infants, the gut microbiota is dominated by Bifidobacterium spp.. Cutibacterium avidum is among the initial colonizers, however, the phylogenetic relationship of infant fecal isolates to isolates from other body sites, and C. avidum carbon utilization related to the infant gut ecosystem have been little investigated.In this study, we investigated the phylogenetic and phenotypic diversity of 28 C. avidum strains, including 16 strains isolated from feces of healthy infants. We investigated the in vitro capacity of C. avidum infant isolates to degrade and consume carbon sources present in the infant gut, and metabolic interactions of C. avidum with infant associated Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum.Isolates of C. avidum showed genetic heterogeneity. C. avidum consumed d- and l-lactate, glycerol, glucose, galactose, N-acetyl-d-glucosamine and maltodextrins. Alpha-galactosidase- and β-glucuronidase activity were a trait of a group of non-hemolytic strains, which were mostly isolated from infant feces. Beta-glucuronidase activity correlated with the ability to ferment glucuronic acid. Co-cultivation with B. infantis and B. bifidum enhanced C. avidum growth and production of propionate, confirming metabolic cross-feeding.This study highlights the phylogenetic and functional diversity of C. avidum, their role as secondary glycan degraders and propionate producers, and suggests adaptation of a subpopulation to the infant gut. |
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| Keywords: | Infant gut microbiota Cross-feeding Glucuronic acid |
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