Bradyrhizobium nanningense sp. nov., Bradyrhizobium guangzhouense sp. nov. and Bradyrhizobium zhanjiangense sp. nov., isolated from effective nodules of peanut in Southeast China |
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| Affiliation: | 1. State Key Lab of Agrobiotechnology, Ministry of Agriculture Key Lab of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China;2. Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, 11340 Mexico D. F., Mexico;3. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain;2. Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain;1. Centro de Biotecnología y Genómica de Plantas (CBGP) and Departamento de Biotecnología (ETSI Agrónomos), Campus de Montegancedo, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, Madrid, Spain;2. VAERSA, Centro para la Investigación y Experimentación Forestal (CIEF), Servicio de Vida Silvestre, Conselleria de Infraestructuras, Territorio y Medio Ambiente, Generalitat Valenciana. Avda. Comarques del Pais Valencià, 114, 46930 Quart de Poblet, Valencia, Spain;3. Microbiología, Departament de Biología, Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain;4. CSIC, Spain;1. Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil;2. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), SBN, Quadra 2, Bloco L, Lote 06, Edifício Capes, 70.040-020, Brasília, Distrito Federal, Brazil;3. Conselho Nacional de Desenvolvimento Científico e Tecnológico, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, 71605-001, Brasília, Distrito Federal, Brazil;1. State Key Laboratory of Agro-Biotechnology; College of Biological Sciences and Rhizobium Research Center, China Agricultural University; MOA Key Laboratory of Soil Microbiology; Beijing 100193, PR China;2. Shandong Shofine Seed Technology Co. Ltd., Jiaxiang 272400, PR China;3. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;4. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, Mexico;5. Engineering Research Centre of Plant Growth Regulators, Ministry of Education; College of Agronomy and Biotechnology, China Agricultural University; Beijing 100193, PR China;6. College of Life Science & Food Engineering, Yibin University, Yibin 644000, Sichuan Province, PR China;1. Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France;2. Laboratory of Microbiology, Dept. Biochemistry and Microbiology, Ghent University, Ghent, Belgium;3. Plateforme MGX, c/o Institut de Genomique Fonctionnelle, Montpellier, 141 rue de la Cardonille, 34 094 Montpellier Cedex, France;1. Department of Crop Sciences, Tshwane University of Technology, South Africa;2. Department of Chemistry, Tshwane University of Technology, South Africa |
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| Abstract: | Nine slow-growing rhizobia isolated from effective nodules on peanut (Arachis hypogaea) were characterized to clarify the taxonomic status using a polyphasic approach. They were assigned to the genus Bradyrhizobium on the basis of 16S rRNA sequences. MLSA of concatenated glnII-recA-dnaK genes classified them into three species represented by CCBAU 53390T, CCBAU 51670T and CCBAU 51778T, which presented the closest similarity to B. guangxiense CCBAU 53363T, B. guangdongense CCBAU 51649T and B. manausense BR 3351T, B. vignae 7-2T and B. forestalis INPA 54BT, respectively. The dDDH (digital DNA-DNA hybridization) and ANI (Average Nucleotide Identity) between the genomes of the three representative strains and type strains for the closest Bradyrhizobium species were less than 42.1% and 91.98%, respectively, below the threshold of species circumscription. Effective nodules could be induced on peanut and Lablab purpureus by all representative strains, while Vigna radiata formed effective nodules only with CCBAU 53390T and CCBAU 51778T. Phenotypic characteristics including sole carbon sources and growth features supported the phylogenetic results. Based on the genotypic and phenotypic features, strains CCBAU 53390T, CCBAU 51670T and CCBAU 51778T are designated the type strains of three novel species, for which the names Bradyrhizobium nanningense sp. nov., Bradyrhizobium guangzhouense sp. nov. and Bradyrhizobium zhanjiangense sp. nov. are proposed, respectively. |
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| Keywords: | Peanut Rhizobia Phylogeny Genome Phenotype |
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