Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage |
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| Institution: | 1. Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280, Plouzané, France;2. Portland State University, Department of Biology and Center for Life in Extreme Environments, P.O. Box 751, Portland, OR 97207, USA;1. Department of Aquatic Animals Disease, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey;2. Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain;3. Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey;4. Institut Mediterrani d’Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain;1. Tampere University, Faculty of Engineering and Natural Sciences, P.O. Box 541, FI-33104, Tampere, Finland;2. Department of Civil, Architectural and Environmental Engineering, University of Napoli Federico II, Via Claudio, 21, 80125, Napoli, Italy;3. INRAE, Univ Montpellier, LBE, Narbonne, France;1. Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INP, UPS, Toulouse, France;2. Université Paris-Saclay, INRAE, PRocédés biOtechnologiques au Service de l’Environnement, 92761 Antony, France;1. School of Natural and Environmental Sciences, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK;2. Departamento de Microbiología y Genética, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain;3. Department of Bioengineering, Faculty of Engineering and Architecture, Sinop University, 57000, Nasuhbasoglu-Sinop, Turkey;4. Science Teaching Programme, Gazi Faculty of Education, Gazi University, Ankara, Turkey;5. IRNASA-CSIC, Salamanca, Spain;6. Department of Molecular Biology and Genetic, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey |
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| Abstract: | A novel moderately thermophilic, heterotrophic anaerobe, designated strain LG1T, was isolated from the Mariner deep-sea hydrothermal vent field along the Eastern Lau Spreading Center and Valu Fa Ridge. Cells of strain LG1T were motile rods, occurring singly or in pairs, 0.6 μm in width and 1.2 μm in length. The strain LG1T grew between 40 and 70 °C (optimum 50–55 °C), at a pH between 5 and 8 (optimum pH 6.5) and with 7.5–50 g L−1 NaCl (optimum 30 g L−1). Sulfur, cystine and thiosulfate were reduced to sulfide, and cell yield was improved in the presence of cystine. Strain LG1T was an organotroph able to use a variety of organic compounds. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain LG1T was affiliated to the genus Marinitoga within the order Petrotogales. It shared 95.34–96.31% 16S rRNA gene sequence similarity with strains of other Marinitoga species, and is most closely related to Marinitoga okinawensis. Genome analysis revealed the presence of a prophage sharing high sequence homology with the viruses MPV1, MCV1 and MCV2 hosted by Marinitoga strains. Based on the data from the phylogenetic analyses and the physiological properties of the novel isolate, we propose that strain LG1T is a representative of a novel species, for which the name Marinitoga lauensis sp. nov. is proposed; the type strain is LG1T (=DSM 106824 = JCM 32613). |
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| Keywords: | Deep-sea hydrothermal vent Lau Basin |
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