Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces |
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Authors: | Baltasar Lucendo-Villarin Kate Cameron Dagmara Szkolnicka Paul Travers Ferdous Khan Jeffrey G. Walton John Iredale Mark Bradley David C. Hay |
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Affiliation: | 1.MRC Centre for Regenerative Medicine, University of Edinburgh;2.School of Chemistry, University of Edinburgh;3.MRC Centre for Inflammation Research, University of Edinburgh |
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Abstract: | Currently, one of the major limitations in cell biology is maintaining differentiated cell phenotype. Biological matrices are commonly used for culturing and maintaining primary and pluripotent stem cell derived hepatocytes. While biological matrices are useful, they permit short term culture of hepatocytes, limiting their widespread application. We have attempted to overcome the limitations using a synthetic polymer coating. Polymers represent one of the broadest classes of biomaterials and possess a wide range of mechanical, physical and chemical properties, which can be fine-tuned for purpose. Importantly, such materials can be scaled to quality assured standards and display batch-to-batch consistency. This is essential if cells are to be expanded for high through-put screening in the pharmaceutical testing industry or for cellular based therapy. Polyurethanes (PUs) are one group of materials that have shown promise in cell culture. Our recent progress in optimizing a polyurethane coated surface, for long-term culture of human hepatocytes displaying stable phenotype, is presented and discussed. |
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Keywords: | Chemistry Issue 91 Pluripotent stem cell polyurethane polymer coating p450 metabolism stable phenotype gamma irradiation ultraviolet irradiation. |
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