Effect of air desiccation and salt stress factors on in vitro regeneration of
rice (Oryza sativa L.) |
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Authors: | Abu Baker Siddique Israt Ara S M Shahinul Islam Narendra Tuteja |
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Institution: | 1.Plant Genetic Engineering Lab; Institute of
Biological Sciences; University of Rajshahi; Rajshahi, Bangladesh;2.Plant Molecular Biology Group; International
Center for Genetic Engineering and Biotechnology; Aruna Asaf
Ali Marg; New Delhi, India |
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Abstract: | Enhancement of callus induction and its regeneration efficiency through in
vitro techniques has been optimized for 2 abiotic stresses (salt and air
desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI
dhan47. The highest frequency of callus induction was obtained for BRRI dhan32
(64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL−1) and Kin
(1.0 mgL−1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7
gL−1) were used and its effect was recorded on the basis of viability
of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content
(RWC). It was observed that in all cases BRRI dhan47 showed highest performance on
tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC
(10.23%) with 11.7 gL−1 NaCl. Plant regeneration capability was
recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h.
In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks
old calli were desiccated for 45 h. It was observed that air desiccation was 2-3
folds more effective for enhancing green plantlet regeneration compared to controls.
Furthermore, desiccated calli also showed the better capability to survive in NaCl induced
abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7
gL−1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed
that the genotypes, air desiccation and NaCl had significant effect on plant regeneration
at P < 0.01. |
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Keywords: | abiotic stress Bangladeshi Indica rice cultivars callus induction mature embryos Oryza sativa plant regeneration |
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