Identification and molecular characterization of the cDNA encoding Cucumis melo allergen,Cuc m 3, a plant pathogenesis-related protein |
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| Authors: | Mojtaba Sankian Jafar Hajavi Malihe Moghadam Abdol-Reza Varasteh |
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| Institution: | 1.Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.;2.Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. |
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| Abstract: | Background:Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which revealed a 456 base-pair (bp) fragment encoding a 151-amino acid polypeptide with a predicted molecular mass of 16.97 kDa, and identified 79 and 178 bp untranslated sequences at the 5′ and 3´ ends, respectively. Results:In silico analysis showed strong similarities between Cuc m 3 and other plant pathogen-related protein 1s from cucumber, grape, bell pepper, and tomato. Conclusion:Here we report the identification and characterization of the Cuc m 3 cDNA, which will be utilized for further analyses of structural and allergenic features of this allergen. Key Words: Melon, allergen, Cuc m 3, plant pathogenesis-related protein 1 |
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