花生幼叶不定芽诱导与快速繁殖

以花生品种‘粤油7号’6~8 d龄幼叶为外植体进行植株再生研究。结果表明,外植体在MS+0.6 mg/L NAA+8 mg/L 6-BA+1 mg/L AgNO₃+3 mg/L Gln培养基上,诱导不定芽效果好,经两次继代培养出芽率达81.03%,每个外植体平均出芽数达5.79个。经伸长培养基MS+2 mg/L 6-BA+4 mg/L GA₃培养,不定芽伸长长度达1.0~2.0 cm。试管苗在培养基1/2 MS+0.5 mg/L NAA+3 mg/L IBA上生根率达86.15%,不定根粗而长,有侧根,移栽成活率达90%,结实率100%。

Adventitious Buds Induction and Rapid Propagation from Leaflet Explants of Arachis hypogaea

The leaflet were used as explants to study plant regeneration,and the results showed that the adventitious bud differentiation could be effectively induced from the explants on medium MS+0.6 mg/L NAA+8 mg/L 6-BA+1 mg/L AgNO₃+3 mg/L Gln,and shoot induction rate and average number of shoots per explant was 81.03% and 5.79 respectively.The optimum medium for adventitions bud elongation was MS+2 mg/L 6-BA+4 mg/L GA₃,and the average elongation length was 1.0-2.0 cm.The rooting rate of explants on 1/2 MS+0.5 mg/L NAA+3 mg/L IBA was 86.15%,and the root was stronger,longer and had more lateral roots.After hardening-off,the survival rate and maturing rate reach to 90% and 100% respectively.