Effects of germanium dioxide,an inhibitor of diatom growth,on the microscopic laboratory cultivation stage of the kelp, <Emphasis Type="Italic">Laminaria saccharina</Emphasis>

The kelp, Laminaria saccharina, is an economically and biologically valuable seaweed used in integrated multi-trophic aquaculture. The development of the initial microscopic phase of the life cycle of this species is carried out in the laboratory. A treatment of germanium dioxide (GeO₂) can be applied to algal laboratory cultures to inhibit the growth of contaminating diatoms. Previous studies reported inhibitory effects also affecting the macroscopic stage of the life cycle of L. saccharina, the sporophyte, but the effects on the microscopic gametophytic life stage are unknown. To determine the effects, cultures of L. saccharina were treated with varying concentrations of GeO₂ and the resulting lengths and surface area of the juvenile sporophytes produced were measured. It was determined that GeO₂ follows a typical dose-effect pattern, increasing the growth rate of juvenile sporophytes until a critical point at which an inhibitory effect was observed. To obtain juvenile sporophytes ready for deployment to aquaculture sites in the shortest time and to successfully reduce diatom contamination, it was determined that a concentration between 0.10 and 0.50mL of GeO₂ per litre of seawater, applied at day 8, was the most efficient.