J-chain expression in human cells producing IgG subclasses |
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| Authors: | P L Haber J Mestecky |
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| Affiliation: | 1. Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642, USA;2. Department of Anesthesiology, University of Rochester, Rochester, NY 14642, USA;3. Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada;1. Department of Internal Medicine II, University Medical Center Regensburg, Regensburg, Germany;2. Clinic of Internal Medicine, Klinikum St. Marien Amberg, Amberg, Germany;3. Center of Clinical Studies, University Medical Center Regensburg, Regensburg, Germany;4. Clinic of Pneumology, University Hospital of Zurich, Zurich, Switzerland;5. Center for Interdisciplinary Sleep Research, University of Zurich, Zurich, Switzerland;1. Department of Neuroscience, University of Rochester Medical Center, 601 Elmwood Ave, Box 603, Rochester, NY 14642, United States;2. Department of Anesthesiology, University of Rochester Medical Center, 601 Elmwood Ave, Box 604, Rochester, NY 14642, United States;3. Department of Chemistry and Biomolecular Sciences, University of Ottawa, D’lorio Hall, 10 Marie Curie, Ottawa, Ontario K1N 6N5, Canada;3. From the Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands,;4. Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, FIN-00290 Helsinki, Finland,;5. Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands,;6. Department of Nephrology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands, and;1. Department of Pathology, Harbin Medical University Cancer Hospital, Harbin, People''s Republic of China;2. Department of Pathological Diagnostics, Yamagata University Faculty of Medicine, Yamagata, Japan;3. Department of Pathology, The First Affiliated Hospital of Hei Longjiang University of Chinese Medicine, Harbin, People''s Republic of China;4. Department of Radiology, Harbin Medical University Cancer Hospital, Harbin, People''s Republic of China;5. Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, People''s Republic of China;6. Department of Pathology, Harbin Medical University, Harbin, People''s Republic of China;7. Department of Otolaryngology, Head and Neck Surgery, Yamagata University Faculty of Medicine, Yamagata, Japan |
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| Abstract: | Previous studies have demonstrated that J chain is expressed not only in cells that produce polymeric immunoglobulins, but also in those engaged in synthesis of monomers including IgG and IgD. The presence of J chain in these cells suggested that its role may not be restricted to the formation of polymers. For the present study, fluorochrome-labeled polyclonal anti-J-chain and monoclonal antibodies to IgG subclasses were used to determine the distribution of J chain in IgG plasma cells from normal human tissues and from pokeweed mitogen (PWM)-stimulated human peripheral blood lymphocytes. The results indicate that J chain is not equally distributed among cells producing different IgG subclasses. The percentages of PWM-stimulated cells containing J chain were: 22 +/- 5 (SE) for IgG1, 49 +/- 6 for IgG2, 17 +/- 7 for IgG3, and 64 +/- 11 for IgG4. Examination of sections of various human lymphoid tissues revealed that the frequency of IgG cells that coexpressed J chain was lower than that observed in the PWM system and displayed variable distribution among IgG subclasses. The frequency of J-chain expression in IgG-producing cells may be related to the degree of cellular maturation and may differ according to the origin of cells. |
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