The ATP binding cassette transporter A1 contributes to the secretion of interleukin 1beta from macrophages but not from monocytes |
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Authors: | Zhou Xiaoqin Engel Thomas Goepfert Christian Erren Michael Assmann Gerd von Eckardstein Arnold |
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Affiliation: | Institute of Arteriosclerosis Research, Domagkstrasse 3, Münster, D-48149, Germany. |
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Abstract: | Deficiency of ABCA1 causes high density lipoprotein deficiency and macrophage foam cell formation in Tangier disease. ABCA1 was also postulated to mediate the secretion of IL-1beta from monocytes and macrophages. We investigated the contribution of ABCA1 to IL-1beta secretion from human monocytes and macrophages of normal donors and Tangier disease patients. Neither an anti-ABCA1 antisense oligonucleotide nor ABCA1 deficiency interfered with LPS-induced secretion of IL-1beta from full blood or freshly isolated monocytes. By contrast, anti-ABCA1 antisense oligonucleotides decreased the LPS-induced secretion of IL-beta from macrophages by 30-50%. The secretion of the precursor pro-IL-1beta and TNFalpha was not inhibited. Compared to normal macrophages, LPS-stimulated Tangier disease macrophages secreted less IL-1beta relative to TNFalpha. Also the spontaneous secretion of IL-1beta by Tangier macrophages was lower than by control cells. We conclude that IL-1beta is secreted from monocytes by an ABCA1-independent pathway and from macrophages by ABCA1-dependent and -independent pathways. |
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Keywords: | monocytes and macrophages cytokines molecular biology |
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