Formation of N-branched oligonucleotides as by-products in solid-phase oligonucleotide synthesis |
| |
Authors: | Cazenave Christian Bathany Katell Rayner Bernard |
| |
Affiliation: | INSERM U386; Université Victor Segalen-Bordeaux 2, 33076 Bordeaux Cedex, France. |
| |
Abstract: | During the synthesis of oligonucleotides by the standard phosphoramidite method using 2'-deoxycytidine- derivatized solid support, a side reaction was observed that gave rise to the formation of high molecular weight N-branched oligomers having two identical chains linked to the 3'-terminal 2'-deoxycytidine.Postsynthesis treatment with neat triethylamine trihydrofluoride selectively cleaved the phosphoramidate linkage and converted the N-branched oligomers back to the expected oligonucleotides. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |