酶解压片分离沙田柚胚襄与胚胎发育研究

用２．５％纤维素酶与２．５％果胶酶混合液在３０℃下对已固定的沙田柚（Ｃｉｔｒｕｓｇｒａｎｄｉｓｖａｒ．ｓｈａｔｉｎｙｕＨｏｒｔ．）胚乳酶解６—１０小时，分离了开花前、授粉至受精间隔期、合子休眠期、胚和胚乳发育各期的胶囊．用Ｏｌｙｍｐａｓ相差显微装置进行观察与摄影，在胚囊中显示了沙田柚胚胎发育各主要阶段的结构特征。分析了酶解压片技术在柑桔属植物胚胎发育研究的前景

ISOLATION OF EMBRYO SAC WITH ENZYME-SQUASH TECHNIQUE AND RESEARCH OF EMBRYONIC DEVELOPMENT OF SHATINYU

The ovules from fixed Shatinyu (Citrus grandis var. shotinyu Hort.)were enzymicly macerated in a mixed solution of 2.5% pectinase and 2.5% cellulase at 30℃ for 6-10 hours. The embryo sacs in the stages before blooming, between pollination and fertilization,and of the zygote dormancy, and in various developmental stages of embryo and endosperm, were isolated. By means of OLYMPAS phase contrast microscope, the embryo sacs were observed and photographs were taken.The structural characteristics of every major stages of the embryonic development were shown in the embryo sacs. The prospects of the enzyme. squash technique for studying the embryonic development of citrus were analysed.