Continuous production of acetone and butanol with shear-activated Clostridium acetobutylicum |
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Authors: | A S Afschar K Schaller K Schügerl |
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Institution: | (1) Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300 Braunschweig, Federal Republic of Germany;(2) Institut für Technische Chemie der Universität Hannover, Callinstr. 3, D-3000 Hannover, Federal Republic of Germany |
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Abstract: | Summary When using shear activation of Clostridium acetobutylicum by pumping the cells through capillaries, the cell growth, glucose consumption and product formation rates are considerably increased. Shear-activated continuous cell culture can be used as an inoculum with a welldefined fermentation activity for batch cultures. Different runs of such batch cultivation yield well-reproducible results which could not be obtained from inocula of other cultures or even of heat-shocked spores. The cells can attain a growth rate higher than 1.6 h-1.The shear-activated continous culture growth is affected already at a butanol concentration lower than 1.6 g/l-1. |
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