Functional analysis of the Bacillus subtilis cysK and cysJI genes |
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Authors: | Jan R van der Ploeg Mario Barone Thomas Leisinger |
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Institution: | Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH-8092 Zürich, Switzerland. jvdploeg@zzmk.unizh.ch |
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Abstract: | The function of the Bacillus subtilis cysK and cysJI (previously designated yvgQR) genes, expected to be involved in the assimilatory sulfate reduction pathway, was investigated. A B. subtilis mutant with a deletion in the cysJI genes was unable to use sulfate or sulfite as sulfur source, which confirmed that these genes encode sulfite reductase. A mutant with a transposon insertion in the cysK gene, whose deduced protein sequence showed similarity to cysteine synthases, grew poorly on sulfate and butanesulfonate. A strain in which cysK and yrhA, a cysK paralog, were inactivated was unable to grow with sulfate. Whereas expression of the cysJI genes was induced by sulfate, expression of cysK was repressed both by sulfate and by cysteine. |
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Keywords: | Sulfate Cysteine biosynthesis Sulfite reductase Cysteine synthase Bacillus subtilis |
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