Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon (Salmo salar) |
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| Authors: | Olafsson Kristinn Hjorleifsdottir Sigridur Pampoulie Christophe Hreggvidsson Gudmundur Oli Gudjonsson Sigurdur |
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| Institution: | Faculty of Life and Environmental Sciences, University of Iceland, Askja, Sturlugta, 101 Reykjavik, Iceland Matís-Prokaria, Gylfaflot 5, 112 Reykjavik, Iceland Marine Research Institute, Skulagata 4, 101 Reykjavik, Iceland Freshwater Institute of Iceland, Keldnaholt, 112 Reykjavik, Iceland. |
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| Abstract: | We report the development of new PCR assays and loading panels for the Atlantic salmon using 15 microsatellite loci. A total of 8, 3 and 4 loci were coamplified in three separate PCRs using labelled primers and loaded on the ABI DNA analyzer in two separate panels. Amplified alleles were clearly typed, and easily interpretable results were obtained. The method was successfully applied in different laboratories, even when different types of DNA polymerase were employed. The method is useful for analysing paternity, population genetics and conservation as well as for selective breeding programmes. |
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| Keywords: | Atlantic salmon conservation genotyping microsatellite |
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